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アイテム
Purification Method of Extracellular Vesicles Derived from Human T-Cell Leukemia Virus Type 1-Infected Cells without Virions
http://hdl.handle.net/10458/0002001138
http://hdl.handle.net/10458/00020011382f826337-233b-498c-ab41-a620feb9bad5
| 名前 / ファイル | ライセンス | アクション |
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| アイテムタイプ | 学術雑誌論文 / Journal Article(1) | |||||||||||||||
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| 公開日 | 2025-03-31 | |||||||||||||||
| タイトル | ||||||||||||||||
| タイトル | Purification Method of Extracellular Vesicles Derived from Human T-Cell Leukemia Virus Type 1-Infected Cells without Virions | |||||||||||||||
| 言語 | en | |||||||||||||||
| 言語 | ||||||||||||||||
| 言語 | eng | |||||||||||||||
| キーワード | ||||||||||||||||
| 言語 | en | |||||||||||||||
| キーワード | Tim-4 affinity purification | |||||||||||||||
| キーワード | ||||||||||||||||
| 言語 | en | |||||||||||||||
| キーワード | extracellular vesicles | |||||||||||||||
| キーワード | ||||||||||||||||
| 言語 | en | |||||||||||||||
| キーワード | genomic RNA | |||||||||||||||
| キーワード | ||||||||||||||||
| 言語 | en | |||||||||||||||
| キーワード | human T-cell leukemia virus type 1 | |||||||||||||||
| キーワード | ||||||||||||||||
| 言語 | en | |||||||||||||||
| キーワード | virions | |||||||||||||||
| 資源タイプ | ||||||||||||||||
| 資源タイプ | journal article | |||||||||||||||
| アクセス権 | ||||||||||||||||
| アクセス権 | open access | |||||||||||||||
| 著者 |
Kawano, Katsumi
× Kawano, Katsumi
× Hashikura, Yuki
× 梅北, 邦彦
WEKO
21827
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| 抄録 | ||||||||||||||||
| 内容記述タイプ | Abstract | |||||||||||||||
| 内容記述 | To mediate intercellular communication, cells produce extracellular vesicles (EVs). These EVs transport many biomolecules such as proteins, nucleic acids, and lipids between cells and regulate pathophysiological actions in the recipient cell. However, EVs and virus particles produced from virus-infected cells are of similar size and specific gravity; therefore, the separation and purification of these two particles is often controversial. When analyzing the physiological functions of EVs from virus-infected cells, the presence or absence of virus particle contamination must always be verified. The human T-cell leukemia virus type 1 (HTLV-1)-infected cell line, MT-2, produces EVs and virus particles. Here, we validated a method for purifying EVs from MT-2 cell culture supernatants while avoiding HTLV-1 viral particle contamination. EV fractions were collected using a combination of immunoprecipitation with Tim-4, which binds to phosphatidylserine, and polymer precipitation. The HTLV-1 viral envelope protein, gp46, was not detected in the EV fraction. Proteomic analysis revealed that EV-constituted proteins were predominant in this EV fraction. Furthermore, the EVs were found to contain the HTLV-1 viral genome. The proposed method can purify EVs while avoiding virus particle contamination and is expected to contribute to future research on EVs derived from HTLV-1-infected cells. | |||||||||||||||
| 言語 | en | |||||||||||||||
| 内容記述 | ||||||||||||||||
| 内容記述タイプ | Other | |||||||||||||||
| 内容記述 | Citation: Kawano, K.; Hashikura, Y.; Umekita, K. Purification Method of Extracellular Vesicles Derived from Human T-Cell Leukemia Virus Type 1-Infected Cells without Virions. Viruses 2024, 16, 249. https://doi.org/10.3390/v16020249 | |||||||||||||||
| 言語 | en | |||||||||||||||
| bibliographic_information |
en : Viruses 巻 16, 号 2, p. 249, 発行日 2024-02-04 |
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| 出版者 | ||||||||||||||||
| 出版者 | MDPI AG | |||||||||||||||
| 言語 | en | |||||||||||||||
| ISSN | ||||||||||||||||
| 収録物識別子タイプ | EISSN | |||||||||||||||
| 収録物識別子 | 1999-4915 | |||||||||||||||
| item_10001_relation_14 | ||||||||||||||||
| 関連タイプ | isVersionOf | |||||||||||||||
| 識別子タイプ | DOI | |||||||||||||||
| 関連識別子 | https://doi.org/10.3390/v16020249 | |||||||||||||||
| 権利 | ||||||||||||||||
| 権利情報 | © 2024 by the authors. | |||||||||||||||
| 言語 | en | |||||||||||||||
| 出版タイプ | ||||||||||||||||
| 出版タイプ | VoR | |||||||||||||||
