| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2025-03-31 |
| タイトル |
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タイトル |
Generation of a Porcine Cell Line Stably Expressing Pig TMPRSS2 for Efficient Isolation of Swine Influenza Virus |
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言語 |
en |
| 言語 |
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言語 |
eng |
| キーワード |
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言語 |
en |
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キーワード |
PK-15 cell |
| キーワード |
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言語 |
en |
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キーワード |
TMPRSS2 |
| キーワード |
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|
言語 |
en |
|
キーワード |
influenza virus |
| キーワード |
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言語 |
en |
|
キーワード |
isolation and propagation |
| 資源タイプ |
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資源タイプ |
journal article |
| アクセス権 |
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アクセス権 |
open access |
| 著者 |
Tanaka, Yuri L
Shofa, Maya
Butlertanaka, Erika P
Niazi, Ahmad Massoud
平井, 卓哉
WEKO
25970
e-Rad_Researcher
60321668
| ja |
平井, 卓哉
宮崎大学
|
| ja-Kana |
ヒライ, タクヤ
|
| en |
Hirai, Takuya
University of Miyazaki
|
Search repository
目堅, 博久
WEKO
24382
e-Rad_Researcher
90633264
| ja |
目堅, 博久
宮崎大学
|
| ja-Kana |
メカタ, ヒロヒサ
|
| en |
Mekata, Hirohisa
University of Miyazaki
|
Search repository
齊藤, 暁
WEKO
33890
e-Rad_Researcher
30621792
| ja |
齊藤, 暁
宮崎大学
|
| ja-Kana |
サイトウ, アカツキ
|
| en |
Saito, Akatsuki
University of Miyazaki
|
Search repository
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| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
Pigs are important animals for meat production but can carry several zoonotic diseases, including the Japanese encephalitis virus, Nipah virus, and influenza viruses. Several Orthomyxoviridae and Coronavirinae respiratory viruses require cleavage of envelope proteins to acquire viral infectivity and consequently, need a host protease or the addition of exogenous trypsin for efficient propagation. Host TMPRSS2 is a key protease responsible for viral cleavage. Stable expression of human TMPRSS2 in African green monkey-derived Vero cells can enhance the porcine epidemic diarrhea virus. However, considering the narrow host tropism of viruses, a porcine cell line expressing pig TMPRSS2 could be optimal for replicating pig-derived viruses. Herein, we generated and evaluated a pig-derived PK-15 cell line stably expressing pig TMPRSS2. This cell line markedly (>1000-fold) and specifically enhanced the growth of influenza viruses. Furthermore, we demonstrated the usefulness of a PK-15 cell line lacking the Stat2 gene with a stable expression of pig TMPRSS2 for efficient virus isolation from clinical samples in the presence of type I interferons. Therefore, PK-15 cells expressing pig TMPRSS2 could be a valuable and promising tool for virus isolation, vaccine production, and virological studies of TMPRSS2-dependent viruses. |
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言語 |
en |
| 内容記述 |
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内容記述タイプ |
Other |
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内容記述 |
Citation: Yuri L Tanaka, Maya Shofa, Erika P Butlertanaka, Ahmad Massoud Niazi, Takuya Hirai, Hirohisa Mekata, Akatsuki Saito, Generation of a Porcine Cell Line Stably Expressing Pig TMPRSS2 for Efficient Isolation of Swine Influenza Virus. Pathogens. 2023-12-24, 13(1), 18-18, https://doi.org/10.3390/pathogens13010018 |
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言語 |
en |
| bibliographic_information |
en : Pathogens
巻 13,
号 1,
p. 18,
発行日 2023-12-24
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| 出版者 |
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出版者 |
MDPI AG |
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言語 |
en |
| ISSN |
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収録物識別子タイプ |
EISSN |
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収録物識別子 |
2076-0817 |
| item_10001_relation_14 |
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関連タイプ |
isVersionOf |
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識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.3390/pathogens13010018 |
| 権利 |
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権利情報 |
© 2023 by the authors. |
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言語 |
en |
| 出版タイプ |
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出版タイプ |
VoR |
Fulltext (3.3 MB)
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