| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2025-03-26 |
| タイトル |
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タイトル |
A new type of sulfation reaction: C-sulfonation for α,β-unsaturated carbonyl groups by a novel sulfotransferase SULT7A1 |
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言語 |
en |
| 言語 |
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言語 |
eng |
| キーワード |
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言語 |
en |
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キーワード |
SULT |
| キーワード |
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言語 |
en |
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キーワード |
prostaglandin |
| キーワード |
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言語 |
en |
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キーワード |
sulfonation |
| キーワード |
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言語 |
en |
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キーワード |
sulfotransferase |
| キーワード |
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言語 |
en |
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キーワード |
α,β-unsaturated carbonyl |
| 資源タイプ |
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資源タイプ |
journal article |
| アクセス権 |
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アクセス権 |
open access |
| 著者 |
黒木, 勝久
WEKO
35139
e-Rad_Researcher
20647036
| ja |
黒木, 勝久
宮崎大学
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| ja-Kana |
クロギ, カツヒサ
|
| en |
Kurogi, Katsuhisa
University of Miyazaki
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Search repository
榊原, 陽一
WEKO
2805
e-Rad_Researcher
90295197
| ja |
榊原, 陽一
宮崎大学
|
| ja-Kana |
サカキバラ, ヨウイチ
|
| en |
Sakakibara, Yoichi
University of Miyazaki
|
Search repository
橋口, 拓勇
WEKO
20074
e-Rad_Researcher
80826305
| ja |
橋口, 拓勇
宮崎大学
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| ja-Kana |
ハシグチ, タクユウ
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| en |
Hashiguchi, Takuyu
University of Miyazaki
|
Search repository
Kakuta, Yoshimitsu
Kanekiyo, Miho
Teramoto, Takamasa
福島, 剛
WEKO
9884
e-Rad_Researcher
10452913
| ja |
福島, 剛
宮崎大学
|
| ja-Kana |
フクシマ, ツヨシ
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| en |
Fukushima, Tsuyoshi
University of Miyazaki
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Search repository
Bamba, Takeshi
松本, 仁
WEKO
6572
e-Rad_Researcher
90363572
| ja |
松本, 仁
宮崎大学
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| ja-Kana |
マツモト, ジン
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| en |
Matsumoto, Jin
University of Miyazaki
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Search repository
Fukusaki, Eiichiro
片岡, 寛章
WEKO
9886
e-Rad_Researcher
10214321
| ja |
片岡, 寛章
宮崎大学
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| ja-Kana |
カタオカ, ヒロアキ
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| en |
Kataoka, Hiroaki
University of Miyazaki
|
Search repository
水光, 正仁
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| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
Cytosolic sulfotransferases (SULTs) are cytosolic enzymes that catalyze the transfer of sulfonate group to key endogenous compounds, altering the physiological functions of their substrates. SULT enzymes catalyze the O-sulfonation of hydroxy groups or N-sulfonation of amino groups of substrate compounds. In this study, we report the discovery of C-sulfonation of α,β-unsaturated carbonyl groups mediated by a new SULT enzyme, SULT7A1, and human SULT1C4. Enzymatic assays revealed that SULT7A1 is capable of transferring the sulfonate group from 3′-phosphoadenosine 5′-phosphosulfate to the α-carbon of α,β-unsaturated carbonyl-containing compounds, including cyclopentenone prostaglandins as representative endogenous substrates. Structural analyses of SULT7A1 suggest that the C-sulfonation reaction is catalyzed by a novel mechanism mediated by His and Cys residues in the active site. Ligand-activity assays demonstrated that sulfonated 15-deoxy prostaglandin J2 exhibits antagonist activity against the prostaglandin receptor EP2 and the prostacyclin receptor IP. Modification of α,β-unsaturated carbonyl groups via the new prostaglandin-sulfonating enzyme, SULT7A1, may regulate the physiological function of prostaglandins in the gut. Discovery of C-sulfonation of α,β-unsaturated carbonyl groups will broaden the spectrum of potential substrates and physiological functions of SULTs. |
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言語 |
en |
| 内容記述 |
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内容記述タイプ |
Other |
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内容記述 |
Citation: Katsuhisa Kurogi, Yoichi Sakakibara, Takuyu Hashiguchi, Yoshimitsu Kakuta, Miho Kanekiyo, Takamasa Teramoto, Tsuyoshi Fukushima, Takeshi Bamba, Jin Matsumoto, Eiichiro Fukusaki, Hiroaki Kataoka, Masahito Suiko, A new type of sulfation reaction: C-sulfonation for α,β-unsaturated carbonyl groups by a novel sulfotransferase SULT7A1, PNAS Nexus, 3(3), 2024-02-29, https://doi.org/10.1093/pnasnexus/pgae097 |
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言語 |
en |
| bibliographic_information |
en : PNAS Nexus
巻 3,
号 3,
p. 97,
発行日 2024-02-29
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| 出版者 |
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出版者 |
Oxford University Press (OUP) |
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言語 |
en |
| ISSN |
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収録物識別子タイプ |
EISSN |
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収録物識別子 |
2752-6542 |
| item_10001_relation_14 |
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関連タイプ |
isVersionOf |
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識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.1093/pnasnexus/pgae097 |
| 権利 |
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権利情報 |
© The Author(s) 2024. |
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言語 |
en |
| 出版タイプ |
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出版タイプ |
VoR |
article (1.3 MB)
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