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Dynamics of the thymic transcriptome at stages of acute thymic involution in Japanese Black calves with a poor prognosis
http://hdl.handle.net/10458/0002001051
http://hdl.handle.net/10458/0002001051a3ef5904-4d92-4346-b389-9fb287546021
| 名前 / ファイル | ライセンス | アクション |
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article (3.4 MB)
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| アイテムタイプ | 学術雑誌論文 / Journal Article(1) | |||||||||||||||||||||
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| 公開日 | 2025-03-25 | |||||||||||||||||||||
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| タイトル | Dynamics of the thymic transcriptome at stages of acute thymic involution in Japanese Black calves with a poor prognosis | |||||||||||||||||||||
| 言語 | en | |||||||||||||||||||||
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| 言語 | eng | |||||||||||||||||||||
| キーワード | ||||||||||||||||||||||
| 言語 | en | |||||||||||||||||||||
| キーワード | Acute thymic involution | |||||||||||||||||||||
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| 言語 | en | |||||||||||||||||||||
| キーワード | Inflammatory response | |||||||||||||||||||||
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| 言語 | en | |||||||||||||||||||||
| キーワード | Thymocyte proliferation | |||||||||||||||||||||
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| 言語 | en | |||||||||||||||||||||
| キーワード | Transcriptome | |||||||||||||||||||||
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| 資源タイプ | journal article | |||||||||||||||||||||
| アクセス権 | ||||||||||||||||||||||
| アクセス権 | open access | |||||||||||||||||||||
| 著者 |
Furukawa, Ryogo
× Furukawa, Ryogo
× 脇谷, 晶一
WEKO
35521
× Kawabata, Risako
× 保田, 昌宏
WEKO
2737
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| 内容記述タイプ | Abstract | |||||||||||||||||||||
| 内容記述 | Transcriptome analysis was performed on the thymus of Japanese Black calves that were necropsied due to poor prognosis, to characterize changes associated with acute thymic involution. Gene expression profiles obtained by DNA microarray analysis of eight calf thymuses were classified into three patterns that correlated with the histopathological stage of acute thymic involution. Using principal component analysis, the first principal component of the global gene expression levels in the calf thymus was associated with the stage of acute thymic involution, suggesting that histopathological changes greatly influence the gene expression profile. Gene ontology enrichment analysis revealed that genes related to cell proliferation, wound healing, and inflammatory responses were the main contributors to the first principal component. Real-time RT-PCR showed that the thymus had lower expression of PCNA, KIFC1, and HES6, and higher expression of SYNPO2, PDGFRB, and TWIST1 during acute thymic involution. Immunohistochemistry demonstrated a decrease in the rate of Ki67-positive cells in the thymic cortex during the late stage of acute thymic involution. The rate of cleaved caspase-1-positive cells increased in the thymic cortex at an earlier stage than the increase in the rate of cleaved caspase-3-positive cells. Vimentin, which was almost absent in the non-involuted thymic cortex, appeared in the thymic cortex during acute thymic involution. These results suggest that in farmed calves with a poor prognosis, inflammatory responses and impaired thymocyte proliferation are primarily involved in acute thymic involution. | |||||||||||||||||||||
| 言語 | en | |||||||||||||||||||||
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| 内容記述タイプ | Other | |||||||||||||||||||||
| 内容記述 | Citation: Ryogo Furukawa, Shoichi Wakitani, Risako Kawabata, Masahiro Yasuda, Dynamics of the thymic transcriptome at stages of acute thymic involution in Japanese Black calves with a poor prognosis, The Veterinary Journal, 307, 106225-106225, 2024-10, https://doi.org/10.1016/j.tvjl.2024.106225 | |||||||||||||||||||||
| 言語 | en | |||||||||||||||||||||
| bibliographic_information |
en : The Veterinary Journal 巻 307, p. 106225 |
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| 出版者 | Elsevier BV | |||||||||||||||||||||
| 言語 | en | |||||||||||||||||||||
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| 収録物識別子タイプ | PISSN | |||||||||||||||||||||
| 収録物識別子 | 1090-0233 | |||||||||||||||||||||
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| 関連タイプ | isVersionOf | |||||||||||||||||||||
| 識別子タイプ | DOI | |||||||||||||||||||||
| 関連識別子 | https://doi.org/10.1016/j.tvjl.2024.106225 | |||||||||||||||||||||
| 権利 | ||||||||||||||||||||||
| 権利情報 | © 2024 The Authors. | |||||||||||||||||||||
| 言語 | en | |||||||||||||||||||||
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| 出版タイプ | VoR | |||||||||||||||||||||
