{"created":"2023-12-18T06:36:41.231152+00:00","id":2000385,"links":{},"metadata":{"_buckets":{"deposit":"911ef0dd-c4f9-4786-8a7f-c61d8a58bdb7"},"_deposit":{"created_by":9,"id":"2000385","owner":"9","owners":[9],"pid":{"revision_id":0,"type":"depid","value":"2000385"},"status":"published"},"_oai":{"id":"oai:miyazaki-u.repo.nii.ac.jp:02000385","sets":["76","76:64"]},"author_link":["34870","9783","34645","34647","26954","26958"],"control_number":"2000385","item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"6","bibliographicPageEnd":"396","bibliographicPageStart":"391","bibliographicVolumeNumber":"12","bibliographic_titles":[{"bibliographic_title":"Cancer Genomics Proteomics","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Background/Aim: Up-regulation of caveolin (CAV)-1 is associated with aggressive prostate cancer. Recently, it has been inferred that CAV2, a co-factor sub-type of CAV1, cross-talks with CAV1 and promotes tumor growth. We previously reported that plasma CAV1 levels are elevated in patients with castration-resistant prostate cancer (CRPC), but not in hormone-sensitive prostate cancer (non-CRPC), implying that CAV1 may be a therapeutic target for CRPC. However, a correlation of CAV1 and CAV2 expression in PC has not yet been reported. Herein, we analyzed associations between PC progression and plasma CAV1 and -2 in Japanese men, and expression of CAV1 and -2 in PC3 (CRPC) and LNCaP (non-CRPC) cell lines. Materials and Methods: We investigated plasma samples from 36 patients with CRPC and 22 with non-CRPC. We used enzyme-linked immunosorbent assay (ELISA) to determine plasma levels of CAV1 and -2, and examined correlations with clinicopathological characteristics such as Gleason grade and clinical T stage. Real-time quantitative reverse transcription-polymerase chain reaction (qRT-PCR) was used to evaluate CAV1 and CAV2 mRNA in PC cell lines. We also introduced CAV1- and CAV2-specific small interfering (siRNA) into PC3 cells to knock-down (KD) both molecules, and examined its influence on the expression of these genes between PC3 CAV1 and -2 KD cells and control cells. Results: Plasma CAV1 and -2 levels in patients with CRPC were significantly higher than in those with non-CRPC (CAV1, p=0.003; CAV2, p<0.001). Plasma levels of CAV1 and -2 were significantly correlated (p<0.001). However, we did not find any significant relationship between CAV1 or CAV2 expression and clinicopathological factors. ELISA and real-time qRT-PCR showed that both proteins and mRNAs in PC3 cells were significantly over-expressed compared to LNCaP cells (p<0.001). In PC3 CAV1 KD cells, expression of CAV2 was suppressed and confirmed the linkage of CAV2 KD and suppression of CAV1 expression. Conclusion: There was a significant correlation between plasma CAV-1 and -2 levels and progression of PC. CAV1 and -2 were highly expressed in the PC3 compared to the LNCaP cell line. Our findings support the potential of these molecules as therapeutic targets for CRPC.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_description_6":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"Citation:\nSugie S, Mukai S, Yamasaki K, Kamibeppu T, Tsukino H, Kamoto T. Significant Association of Caveolin-1 and Caveolin-2 with Prostate Cancer Progression. Cancer Genomics Proteomics. 2015 Nov-Dec;12(6):391-6. PMID: 26543085.","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Cancer & Genome Proteomics","subitem_publisher_language":"en"}]},"item_10001_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"Copyright© 2015, International Institute of Anticancer Research (Dr. John G. Delinasios), All rights reserved","subitem_rights_language":"en"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1109-6535","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"0000000106573887","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"https://isni.org/isni/0000000106573887"}],"affiliationNames":[{"affiliationName":"宮崎大学","affiliationNameLang":"ja"},{"affiliationName":"University of Miyazaki","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"杉江, 悟","creatorNameLang":"ja"},{"creatorName":"スギエ, サトル","creatorNameLang":"ja-Kana"},{"creatorName":"Sugie, Satoru","creatorNameLang":"en"}],"familyNames":[{"familyName":"杉江","familyNameLang":"ja"},{"familyName":"スギエ","familyNameLang":"ja-Kana"},{"familyName":"Sugie","familyNameLang":"en"}],"givenNames":[{"givenName":"悟","givenNameLang":"ja"},{"givenName":"サトル","givenNameLang":"ja-Kana"},{"givenName":"Satoru","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"26954","nameIdentifierScheme":"WEKO"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"0000000106573887","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/0000000106573887"}],"affiliationNames":[{"affiliationName":"宮崎大学","affiliationNameLang":"ja"},{"affiliationName":"University of Miyazaki","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"Mukai, Shoichiro","creatorNameLang":"en"},{"creatorName":"向井, 尚一郎","creatorNameLang":"ja"},{"creatorName":"ムカイ, ショウイチロウ","creatorNameLang":"ja-Kana"},{"creatorName":"Mukai, Syoichiro","creatorNameLang":"en"}],"familyNames":[{"familyName":"Mukai","familyNameLang":"en"},{"familyName":"向井","familyNameLang":"ja"},{"familyName":"ムカイ","familyNameLang":"ja-Kana"},{"familyName":"Mukai","familyNameLang":"en"}],"givenNames":[{"givenName":"Shoichiro","givenNameLang":"en"},{"givenName":"尚一郎","givenNameLang":"ja"},{"givenName":"ショウイチロウ","givenNameLang":"ja-Kana"},{"givenName":"Syoichiro","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"26958","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"山崎, 浩司","creatorNameLang":"ja"},{"creatorName":"ヤマサキ, コウジ","creatorNameLang":"ja-Kana"},{"creatorName":"Yamasaki, Koji","creatorNameLang":"en"}],"familyNames":[{"familyName":"山崎","familyNameLang":"ja"},{"familyName":"ヤマサキ","familyNameLang":"ja-Kana"},{"familyName":"Yamasaki","familyNameLang":"en"}],"givenNames":[{"givenName":"浩司","givenNameLang":"ja"},{"givenName":"コウジ","givenNameLang":"ja-Kana"},{"givenName":"Koji","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"34647","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"上別府, 豊治","creatorNameLang":"ja"},{"creatorName":"カミベップ, トヨハル","creatorNameLang":"ja-Kana"},{"creatorName":"Kamibeppu, Toyoharu","creatorNameLang":"en"}],"familyNames":[{"familyName":"上別府","familyNameLang":"ja"},{"familyName":"カミベップ","familyNameLang":"ja-Kana"},{"familyName":"Kamibeppu","familyNameLang":"en"}],"givenNames":[{"givenName":"豊治","givenNameLang":"ja"},{"givenName":"トヨハル","givenNameLang":"ja-Kana"},{"givenName":"Toyoharu","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"34645","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"月野, 浩昌","creatorNameLang":"ja"},{"creatorName":"ツキノ, ヒロマサ","creatorNameLang":"ja-Kana"},{"creatorName":"Tsukino, Hiromasa","creatorNameLang":"en"}],"familyNames":[{"familyName":"月野","familyNameLang":"ja"},{"familyName":"ツキノ","familyNameLang":"ja-Kana"},{"familyName":"Tsukino","familyNameLang":"en"}],"givenNames":[{"givenName":"浩昌","givenNameLang":"ja"},{"givenName":"ヒロマサ","givenNameLang":"ja-Kana"},{"givenName":"Hiromasa","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"34870","nameIdentifierScheme":"WEKO"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"0000000106573887","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"https://isni.org/isni/0000000106573887"}],"affiliationNames":[{"affiliationName":"宮崎大学","affiliationNameLang":"ja"},{"affiliationName":"University of Miyazaki","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"賀本, 敏行","creatorNameLang":"ja"},{"creatorName":"カモト, トシユキ","creatorNameLang":"ja-Kana"},{"creatorName":"Kamoto, Toshiyuki","creatorNameLang":"en"}],"familyNames":[{"familyName":"賀本","familyNameLang":"ja"},{"familyName":"カモト","familyNameLang":"ja-Kana"},{"familyName":"Kamoto","familyNameLang":"en"}],"givenNames":[{"givenName":"敏行","givenNameLang":"ja"},{"givenName":"トシユキ","givenNameLang":"ja-Kana"},{"givenName":"Toshiyuki","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"9783","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"00281098","nameIdentifierScheme":"e-Rad_Researcher","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=00281098"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2023-12-18"}],"filename":"honbun.pdf","filesize":[{"value":"136 KB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"article","objectType":"fulltext","url":"https://miyazaki-u.repo.nii.ac.jp/record/2000385/files/honbun.pdf"},"version_id":"cfebbee1-8126-434a-834e-3f61c743ec85"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Caveolin-1","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"caveolin-2","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"prostate cancer progression","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Significant Association of Caveolin-1 and Caveolin-2 with Prostate Cancer Progression","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Significant Association of Caveolin-1 and Caveolin-2 with Prostate Cancer Progression","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"9","path":["76","64"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2023-12-18"},"publish_date":"2023-12-18","publish_status":"0","recid":"2000385","relation_version_is_last":true,"title":["Significant Association of Caveolin-1 and Caveolin-2 with Prostate Cancer Progression"],"weko_creator_id":"9","weko_shared_id":-1},"updated":"2025-01-07T05:35:52.818523+00:00"}