{"created":"2023-10-24T04:11:00.597240+00:00","id":2000102,"links":{},"metadata":{"_buckets":{"deposit":"661900d1-675b-4f6d-a2bc-ed53c167e0ef"},"_deposit":{"created_by":9,"id":"2000102","owner":"9","owners":[9],"pid":{"revision_id":0,"type":"depid","value":"2000102"},"status":"published"},"_oai":{"id":"oai:miyazaki-u.repo.nii.ac.jp:02000102","sets":["76","76:64"]},"author_link":["34820","26958","33765","9783","34647","34645"],"control_number":"2000102","item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2018-10-18","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"144","bibliographicPageStart":"135","bibliographicVolumeNumber":"10","bibliographic_titles":[{"bibliographic_title":"Research and Reports in Urology","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Background: Caveolin (Cav)-1 and Cav-2 are cell membrane proteins, which are structural proteins of caveolae and are reported to be positive regulators of cell survival and metastasis in prostate cancer (PC). In a previous study, we reported that elevated levels of Cav-1 and Cav-2 were significantly associated with PC progression. However, their functions in PC have not yet been clarified. In this study, we examined the function of Cav-1 and Cav-2 in PC cell invasiveness and motility.\nMaterials and methods: We introduced Cav-1- and Cav-2-specific small interfering into PC3 cells to knock-down (KD) both molecules. We also performed cell proliferation assay, wound healing assay, migration assay, and invasion assay using PC3 cells and compared the results between Cav-1-KD, Cav-2-KD, and negative control PC3 cells. In addition, we performed real-time quantitative PCR (RT-qPCR) and RT2 Profiler PCR Array analysis to identify factors influencing migration.\nResults: We observed no significant difference in the proliferative and invasive activities of Cav-1-KD and Cav-2-KD PC3 cells; however, the cell motility was significantly decreased compared with negative control PC3 cells. RT-qPCR revealed that the expression of vimentin and N-cadherin was downregulated in Cav-1-KD PC3 cells. In addition, PCR array revealed a decreased expression of MGAT5, MMP13, and MYCL in Cav-1-KD PC3 and ETV4, FGFR4, and SRC in Cav-2-KD PC3.\nConclusion: Cav-1 and Cav-2 may positively contribute to the upregulation of castration-resistant PC cell migration. Cav-induced regulation of several molecules including vimentin, N-cadherin, MGAT5, MMP13, MYCL, ETV4, FGFR4, and SRC may have an important role in PC3 cell motility. However, further examination will be required.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_description_6":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"Citation:\nKamibeppu T, Yamasaki K, Nakahara K, Nagai T, Terada N, Tsukino H, Mukai S, Kamoto T. Caveolin-1 and -2 regulate cell motility in castration-resistant prostate cancer. Res Rep Urol. 2018;10:135-144\nhttps://doi.org/10.2147/RRU.S173377","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Dove Medical Press","subitem_publisher_language":"en"}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.2147/RRU.S173377","subitem_relation_type_select":"DOI"}}]},"item_10001_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2018 The Author(s). This work is published and licensed by Dove Medical Press Limited.","subitem_rights_language":"en"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"上別府, 豊治","creatorNameLang":"ja"},{"creatorName":"カミベップ, トヨハル","creatorNameLang":"ja-Kana"},{"creatorName":"Kamibeppu, Toyoharu","creatorNameLang":"en"}],"familyNames":[{"familyName":"上別府","familyNameLang":"ja"},{"familyName":"カミベップ","familyNameLang":"ja-Kana"},{"familyName":"Kamibeppu","familyNameLang":"en"}],"givenNames":[{"givenName":"豊治","givenNameLang":"ja"},{"givenName":"トヨハル","givenNameLang":"ja-Kana"},{"givenName":"Toyoharu","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"34645","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"山崎, 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コズエ","creatorNameLang":"ja-Kana"},{"creatorName":"Nakahara, Kozue","creatorNameLang":"en"}],"familyNames":[{"familyName":"中原","familyNameLang":"ja"},{"familyName":"ナカハラ","familyNameLang":"ja-Kana"},{"familyName":"Nakahara","familyNameLang":"en"}],"givenNames":[{"givenName":"梢","givenNameLang":"ja"},{"givenName":"コズエ","givenNameLang":"ja-Kana"},{"givenName":"Kozue","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"34820","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Nagai, Takahiro","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Terada, Naoki","creatorNameLang":"en"}],"familyNames":[{"familyName":"Terada","familyNameLang":"en"}],"givenNames":[{"givenName":"Naoki","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"33765","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Tsukino, Hiromasa","creatorNameLang":"en"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"0000000106573887","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/0000000106573887"}],"affiliationNames":[{"affiliationName":"宮崎大学","affiliationNameLang":"ja"},{"affiliationName":"University of Miyazaki","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"Mukai, Shoichiro","creatorNameLang":"en"},{"creatorName":"向井, 尚一郎","creatorNameLang":"ja"},{"creatorName":"ムカイ, ショウイチロウ","creatorNameLang":"ja-Kana"},{"creatorName":"Mukai, Syoichiro","creatorNameLang":"en"}],"familyNames":[{"familyName":"Mukai","familyNameLang":"en"},{"familyName":"向井","familyNameLang":"ja"},{"familyName":"ムカイ","familyNameLang":"ja-Kana"},{"familyName":"Mukai","familyNameLang":"en"}],"givenNames":[{"givenName":"Shoichiro","givenNameLang":"en"},{"givenName":"尚一郎","givenNameLang":"ja"},{"givenName":"ショウイチロウ","givenNameLang":"ja-Kana"},{"givenName":"Syoichiro","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"26958","nameIdentifierScheme":"WEKO"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"0000000106573887","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"https://isni.org/isni/0000000106573887"}],"affiliationNames":[{"affiliationName":"宮崎大学","affiliationNameLang":"ja"},{"affiliationName":"University of Miyazaki","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"賀本, 敏行","creatorNameLang":"ja"},{"creatorName":"カモト, トシユキ","creatorNameLang":"ja-Kana"},{"creatorName":"Kamoto, Toshiyuki","creatorNameLang":"en"}],"familyNames":[{"familyName":"賀本","familyNameLang":"ja"},{"familyName":"カモト","familyNameLang":"ja-Kana"},{"familyName":"Kamoto","familyNameLang":"en"}],"givenNames":[{"givenName":"敏行","givenNameLang":"ja"},{"givenName":"トシユキ","givenNameLang":"ja-Kana"},{"givenName":"Toshiyuki","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"9783","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"00281098","nameIdentifierScheme":"e-Rad_Researcher","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=00281098"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2023-10-24"}],"filename":"rru-173377-caveolin-1-and-2-regulate-cell-motility-in-castration-resist-100218.pdf","filesize":[{"value":"370 KB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"本文","objectType":"fulltext","url":"https://miyazaki-u.repo.nii.ac.jp/record/2000102/files/rru-173377-caveolin-1-and-2-regulate-cell-motility-in-castration-resist-100218.pdf"},"version_id":"73944d1b-82a8-40ee-af05-a244a00088aa"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"caveolin-1","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"caveolin-2","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"CRPC","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"cell motility","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal 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