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A bell‐shaped pattern of urinary aquaporin‐2‐bearing extracellular vesicle release in an experimental model of nephronophthisis
http://hdl.handle.net/10458/0002000098
http://hdl.handle.net/10458/0002000098e676b8db-4b21-4712-9696-ec1b098854f3
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本文 (1.1 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||||||||||||||
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| 公開日 | 2023-10-18 | |||||||||||||||||
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| タイトル | A bell‐shaped pattern of urinary aquaporin‐2‐bearing extracellular vesicle release in an experimental model of nephronophthisis | |||||||||||||||||
| 言語 | en | |||||||||||||||||
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| 言語 | eng | |||||||||||||||||
| キーワード | ||||||||||||||||||
| 言語 | en | |||||||||||||||||
| キーワード | Aquaporin-2 | |||||||||||||||||
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| 言語 | en | |||||||||||||||||
| キーワード | pcy mice | |||||||||||||||||
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| 言語 | en | |||||||||||||||||
| キーワード | polycystic kidney disease | |||||||||||||||||
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| 言語 | en | |||||||||||||||||
| キーワード | urinary extracellular vesicles (exosomes) | |||||||||||||||||
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| 資源タイプ | journal article | |||||||||||||||||
| 著者 |
三小田, 伸之
× 三小田, 伸之× 園田, 紘子
WEKO
5451
× Oshikawa, Sayaka× 星野, 雄也× Matsuzaki, Toshiyuki× 池田, 正浩
WEKO
1081
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| 内容記述タイプ | Abstract | |||||||||||||||||
| 内容記述 | The DBA/2-FG pcy (pcy) mouse is a model of human nephronophthisis, a recessive cystic kidney disease. Renal expression of aquaporin-2 (AQP2), a water channel protein, has been shown to be altered in pcy mice. However, the relationship between the renal expression and its release in urinary extracellular vesicles (uEV-AQP2), which account for most urinary AQP2, in pcy mice has remained largely unknown. In this study, we examined age-related alterations of this relationship in pcy mice. In comparison with control mice, pcy mice after the age of 14 weeks showed defective urinary concentration ability with an increase in urinary volume. Interestingly, the release of uEV-AQP2 increased progressively up to the age of 16 weeks, but at 21 weeks the release did not significantly differ from that in control mice (i.e., a bell-shaped pattern was evident). Similar results were obtained for uEV marker proteins, including tumor susceptibility gene 101 (TSG101) protein and apoptosis-linked gene 2-interacting protein X (Alix). Immunoblot analysis revealed that renal AQP2 expression increased progressively from 11 weeks, and immunohistochemistry showed that this increase was possibly due to an increase in the number of AQP2-positive cells. Analysis of mRNAs for seven types of AQP expressed in the kidney supported this notion. These data suggest that the level of uEV-AQP2 does not simply mirror the renal expression of AQP2 and that the altered release of uEV-AQP2 in pcy mice depends on the numbers of both renal AQP2-positive cells and EVs released into the urine. | |||||||||||||||||
| 言語 | en | |||||||||||||||||
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| 内容記述タイプ | Other | |||||||||||||||||
| 内容記述 | Citation: Mikoda, N., Sonoda, H., Oshikawa, S., Hoshino, Y., Matsuzaki, T., Ikeda, M.. A bell-shaped pattern of urinary aquaporin-2-bearing extracellular vesicle release in an experimental model of nephronophthisis. Physiol Rep, 7 (9), 2019, e14092, https://doi.org/10.14814/phy2.14092 |
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| 言語 | en | |||||||||||||||||
| 書誌情報 |
en : Physiological Reports 巻 7, 号 9, p. e14092, 発行日 2019 |
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| 出版者 | Wiley | |||||||||||||||||
| 言語 | en | |||||||||||||||||
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| 収録物識別子タイプ | ISSN | |||||||||||||||||
| 収録物識別子 | 2051-817X | |||||||||||||||||
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| 関連タイプ | isVersionOf | |||||||||||||||||
| 識別子タイプ | DOI | |||||||||||||||||
| 関連識別子 | https://doi.org/10.14814/phy2.14092 | |||||||||||||||||
| 権利 | ||||||||||||||||||
| 言語 | en | |||||||||||||||||
| 権利情報 | © 2019 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of The Physiological Society and the American Physiological Society. | |||||||||||||||||
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| 出版タイプ | VoR | |||||||||||||||||
