{"created":"2023-09-06T07:54:48.008604+00:00","id":2000046,"links":{},"metadata":{"_buckets":{"deposit":"6e369c51-50ad-456e-8b9f-ec9ee54d5693"},"_deposit":{"created_by":5,"id":"2000046","owner":"5","owners":[5],"pid":{"revision_id":0,"type":"depid","value":"2000046"},"status":"published"},"_oai":{"id":"oai:miyazaki-u.repo.nii.ac.jp:02000046","sets":["76","76:64"]},"author_link":["34112","30100","34061","34113","1864","27796"],"control_number":"2000046","item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2021","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"6","bibliographicPageEnd":"897","bibliographicPageStart":"889","bibliographicVolumeNumber":"83","bibliographic_titles":[{"bibliographic_title":"Journal of Veterinary Medical Science","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Papillomatous digital dermatitis (PDD) is a polymicrobial infection causing lameness \nin dairy cattle. Culture-independent analysis has shown that Treponema phagedenis is present \nconsistently and predominantly in the lesions. However, the pathogenesis of PDD, especially \nthe tissue penetration pathway, has not been examined. In the present study, we investigated \nwhether T. phagedenis strains isolated from PDD produce proteolytic enzyme (s) for disruption of \nthe epithelial cell barrier and have the ability to translocate in polarized normal human epidermal \nkeratinocytes (NHEK) in vitro. Ten strains of T. phagedenis isolated from lesions did not show \nproteolytic activity on modified skim milk agar, although a human strain of T. denticola used as \na control showed such activity. The integrity of tight junctions was monitored by measurement \nof transepithelial electrical resistance (TER). The TER values after inoculation of the T. phagedenis\nstrains examined did not change during the experimental period; however, apical to basolateral \ntranslocation of T. phagedenis was confirmed after 24 hr by microscopy and Treponema-specific \nPCR. We further confirmed that translocation of T. phagedenis was accelerated by co-inoculation \nwith live T. denticola, but not with heat-killed organisms. Furthermore, tight junction ZO-1 protein \nwas not lost intensity after inoculation with T. phagedenis and the organism was observed in \nNHEK cells using a florescence microscope. These results suggest that T. phagedenis strains may \ntranslocate via a transcellular route in vitro and that the invasion is accelerated by other bacteria, \nsuch as T. denticola, producing proteolytic activity.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_description_6":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"Citation: Rathanon KHEMGAEW, Mari OMACHI, Tomoe TAKESADA, Torrung VETCHAPITAK, Hiroyuki SATO, Takako TANIGUCHI, Naoaki MISAWA, Transcellular penetration of Treponema phagedenis isolated from papillomatous digital dermatitis in polarized normal human epidermal keratinocytes in vitro, Journal of Veterinary Medical Science, 2021, Volume 83, Issue 6, Pages 889-897, Released on J-STAGE June 09, 2021, Advance online publication April 14, 2021, Online ISSN 1347-7439, Print ISSN 0916-7250, https://doi.org/10.1292/jvms.21-0034, https://www.jstage.jst.go.jp/article/jvms/83/6/83_21-0034/_article/-char/en","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Japanese Society of Veterinary Science","subitem_publisher_language":"en"}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1292/jvms.21-0034","subitem_relation_type_select":"DOI"}}]},"item_10001_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2021 by the Japanese Society of Veterinary Science","subitem_rights_language":"en"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"09167250","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Khemgaew, Rathanon","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Omachi, Mari","creatorNameLang":"en"}],"familyNames":[{"familyName":"Omachi","familyNameLang":"en"}],"givenNames":[{"givenName":"Mari","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"34112","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Takesada, Tomoe","creatorNameLang":"en"}],"familyNames":[{"familyName":"Takesada","familyNameLang":"en"}],"givenNames":[{"givenName":"Tomoe","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"34113","nameIdentifierScheme":"WEKO"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"Torrung, Vetchapitak","creatorNameLang":"en"},{"creatorName":"トルン, ベッチャピタク","creatorNameLang":"ja"},{"creatorName":"トルン, ベッチャピタク","creatorNameLang":"ja-Kana"}],"familyNames":[{"familyName":"Torrung","familyNameLang":"en"},{"familyName":"トルン","familyNameLang":"ja"},{"familyName":"トルン","familyNameLang":"ja-Kana"}],"givenNames":[{"givenName":"Vetchapitak","givenNameLang":"en"},{"givenName":"ベッチャピタク","givenNameLang":"ja"},{"givenName":"ベッチャピタク","givenNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"34061","nameIdentifierScheme":"WEKO"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"0000000106573887","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"https://isni.org/isni/0000000106573887"}],"affiliationNames":[{"affiliationName":"宮崎大学","affiliationNameLang":"ja"},{"affiliationName":"University of Miyazaki","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"Satoh, Hiroyuki","creatorNameLang":"en"},{"creatorName":"佐藤, 裕之","creatorNameLang":"ja"},{"creatorName":"サトウ, ヒロユキ","creatorNameLang":"ja-Kana"},{"creatorName":"Sato, Hiroyuki","creatorNameLang":"en"}],"familyNames":[{"familyName":"Satoh","familyNameLang":"en"},{"familyName":"佐藤","familyNameLang":"ja"},{"familyName":"サトウ","familyNameLang":"ja-Kana"},{"familyName":"Sato","familyNameLang":"en"}],"givenNames":[{"givenName":"Hiroyuki","givenNameLang":"en"},{"givenName":"裕之","givenNameLang":"ja"},{"givenName":"ヒロユキ","givenNameLang":"ja-Kana"},{"givenName":"Hiroyuki","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"27796","nameIdentifierScheme":"WEKO"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"0000000106573887","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"https://isni.org/isni/0000000106573887"}],"affiliationNames":[{"affiliationName":"宮崎大学","affiliationNameLang":"ja"},{"affiliationName":"University of Miyazaki","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"谷口, 喬子","creatorNameLang":"ja"},{"creatorName":"タニグチ, タカコ","creatorNameLang":"ja-Kana"},{"creatorName":"Taniguchi, Takako","creatorNameLang":"en"}],"familyNames":[{"familyName":"谷口","familyNameLang":"ja"},{"familyName":"タニグチ","familyNameLang":"ja-Kana"},{"familyName":"Taniguchi","familyNameLang":"en"}],"givenNames":[{"givenName":"喬子","givenNameLang":"ja"},{"givenName":"タカコ","givenNameLang":"ja-Kana"},{"givenName":"Takako","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"30100","nameIdentifierScheme":"WEKO"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"0000000106573887","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"https://isni.org/isni/0000000106573887"}],"affiliationNames":[{"affiliationName":"宮崎大学","affiliationNameLang":"ja"},{"affiliationName":"University of Miyazaki","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"三澤, 尚明","creatorNameLang":"ja"},{"creatorName":"ミサワ, ナオアキ","creatorNameLang":"ja-Kana"},{"creatorName":"Misawa, Naoaki","creatorNameLang":"en"}],"familyNames":[{"familyName":"三澤","familyNameLang":"ja"},{"familyName":"ミサワ","familyNameLang":"ja-Kana"},{"familyName":"Misawa","familyNameLang":"en"}],"givenNames":[{"givenName":"尚明","givenNameLang":"ja"},{"givenName":"ナオアキ","givenNameLang":"ja-Kana"},{"givenName":"Naoaki","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1864","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"20229678","nameIdentifierScheme":"e-Rad_Researcher","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=20229678"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2023-09-06"}],"filename":"honbun_Khemgaew.pdf","filesize":[{"value":"2.5 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Fulltext","objectType":"fulltext","url":"https://miyazaki-u.repo.nii.ac.jp/record/2000046/files/honbun_Khemgaew.pdf"},"version_id":"460b78bd-e826-49ac-a510-d19ba309683b"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"dairy cattle","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"normal human epidermal keratinocytes cell","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"papillomatous digital dermatitis","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"translocation","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Treponema phagedenis","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Transcellular penetration of Treponema phagedenis isolated from papillomatous digital dermatitis in polarized normal human epidermal keratinocytes in vitro","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Transcellular penetration of Treponema phagedenis isolated from papillomatous digital dermatitis in polarized normal human epidermal keratinocytes in vitro","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"5","path":["76","64"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2023-09-06"},"publish_date":"2023-09-06","publish_status":"0","recid":"2000046","relation_version_is_last":true,"title":["Transcellular penetration of Treponema phagedenis isolated from papillomatous digital dermatitis in polarized normal human epidermal keratinocytes in vitro"],"weko_creator_id":"5","weko_shared_id":-1},"updated":"2025-01-21T07:19:32.564831+00:00"}