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Transcellular penetration of Treponema phagedenis isolated from papillomatous digital dermatitis in polarized normal human epidermal keratinocytes in vitro
http://hdl.handle.net/10458/0002000046
http://hdl.handle.net/10458/000200004640ab6ece-1491-46b2-8993-2141a2d4ae56
| 名前 / ファイル | ライセンス | アクション |
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||||
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| 公開日 | 2023-09-06 | |||||||
| タイトル | ||||||||
| タイトル | Transcellular penetration of Treponema phagedenis isolated from papillomatous digital dermatitis in polarized normal human epidermal keratinocytes in vitro | |||||||
| 言語 | en | |||||||
| 言語 | ||||||||
| 言語 | eng | |||||||
| キーワード | ||||||||
| 言語 | en | |||||||
| 主題Scheme | Other | |||||||
| キーワード | dairy cattle | |||||||
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| 言語 | en | |||||||
| 主題Scheme | Other | |||||||
| キーワード | normal human epidermal keratinocytes cell | |||||||
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| 言語 | en | |||||||
| 主題Scheme | Other | |||||||
| キーワード | papillomatous digital dermatitis | |||||||
| キーワード | ||||||||
| 言語 | en | |||||||
| 主題Scheme | Other | |||||||
| キーワード | translocation | |||||||
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| 言語 | en | |||||||
| 主題Scheme | Other | |||||||
| キーワード | Treponema phagedenis | |||||||
| 資源タイプ | ||||||||
| 資源タイプ | journal article | |||||||
| アクセス権 | ||||||||
| 著者 |
Khemgaew, Rathanon
× Khemgaew, Rathanon
× Omachi, Mari× Takesada, Tomoe× トルン, ベッチャピタク× 佐藤, 裕之× 谷口, 喬子× 三澤, 尚明 |
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| 抄録 | ||||||||
| 内容記述タイプ | Abstract | |||||||
| 内容記述 | Papillomatous digital dermatitis (PDD) is a polymicrobial infection causing lameness in dairy cattle. Culture-independent analysis has shown that Treponema phagedenis is present consistently and predominantly in the lesions. However, the pathogenesis of PDD, especially the tissue penetration pathway, has not been examined. In the present study, we investigated whether T. phagedenis strains isolated from PDD produce proteolytic enzyme (s) for disruption of the epithelial cell barrier and have the ability to translocate in polarized normal human epidermal keratinocytes (NHEK) in vitro. Ten strains of T. phagedenis isolated from lesions did not show proteolytic activity on modified skim milk agar, although a human strain of T. denticola used as a control showed such activity. The integrity of tight junctions was monitored by measurement of transepithelial electrical resistance (TER). The TER values after inoculation of the T. phagedenis strains examined did not change during the experimental period; however, apical to basolateral translocation of T. phagedenis was confirmed after 24 hr by microscopy and Treponema-specific PCR. We further confirmed that translocation of T. phagedenis was accelerated by co-inoculation with live T. denticola, but not with heat-killed organisms. Furthermore, tight junction ZO-1 protein was not lost intensity after inoculation with T. phagedenis and the organism was observed in NHEK cells using a florescence microscope. These results suggest that T. phagedenis strains may translocate via a transcellular route in vitro and that the invasion is accelerated by other bacteria, such as T. denticola, producing proteolytic activity. |
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| 言語 | en | |||||||
| 内容記述 | ||||||||
| 内容記述タイプ | Other | |||||||
| 内容記述 | Citation: Rathanon KHEMGAEW, Mari OMACHI, Tomoe TAKESADA, Torrung VETCHAPITAK, Hiroyuki SATO, Takako TANIGUCHI, Naoaki MISAWA, Transcellular penetration of Treponema phagedenis isolated from papillomatous digital dermatitis in polarized normal human epidermal keratinocytes in vitro, Journal of Veterinary Medical Science, 2021, Volume 83, Issue 6, Pages 889-897, Released on J-STAGE June 09, 2021, Advance online publication April 14, 2021, Online ISSN 1347-7439, Print ISSN 0916-7250, https://doi.org/10.1292/jvms.21-0034, https://www.jstage.jst.go.jp/article/jvms/83/6/83_21-0034/_article/-char/en | |||||||
| 言語 | en | |||||||
| 書誌情報 |
en : Journal of Veterinary Medical Science 巻 83, 号 6, p. 889-897, 発行日 2021 |
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| 出版者 | ||||||||
| 出版者 | Japanese Society of Veterinary Science | |||||||
| 言語 | en | |||||||
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| 収録物識別子タイプ | ISSN | |||||||
| 収録物識別子 | 09167250 | |||||||
| DOI | ||||||||
| 関連タイプ | isVersionOf | |||||||
| 識別子タイプ | DOI | |||||||
| 関連識別子 | https://doi.org/10.1292/jvms.21-0034 | |||||||
| 権利 | ||||||||
| 言語 | en | |||||||
| 権利情報 | © 2021 by the Japanese Society of Veterinary Science | |||||||
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| 出版タイプ | VoR | |||||||
