{"created":"2023-05-15T09:58:03.000298+00:00","id":1917,"links":{},"metadata":{"_buckets":{"deposit":"e0b5f6a0-a198-43c0-b702-7255858a0eea"},"_deposit":{"created_by":5,"id":"1917","owner":"5","owners":[5],"pid":{"revision_id":0,"type":"depid","value":"1917"},"status":"published"},"_oai":{"id":"oai:miyazaki-u.repo.nii.ac.jp:00001917","sets":["74","74:32"]},"author_link":["2492"],"item_10007_description_13":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"本研究ではアントシアニンのポリアシル化によりハナショウブの花色発現の安定化を促進し、その観賞価値を高めるために、アシル化アントシアニンおよびアントシアニンアシル基転移酵素の特性解明とその酵素遺伝子の分析を行った。得られた成果は以下の通りである。\n1.ハナショウブの品種およびその野生系統は、主要アントシアニンの構成により16種類の型に分類された。これらのうち、非アシル化型としてpetunidin 3RG5G -malvidin 3RG5G、malvidin 3RG5G-peonidin 3RG5G、peonidin 3RG5G-cyanidin 3RG5Gおよびpeonidin 3RG-cyanidin 3RGの4つの型が新たに発見された。\n2.malvidin 3RG5G およびpetunidin 3RG5Gはともに3.2>6.2>5.2>4.2の水溶液pHの順に安定であった。また、両アントシアニンともpH3.2の水溶液中ではかなりの安定性を示したが、pH4.2~6.2の水溶液中では非常に不安定であった。これに対して、malvidin 3RG5Gおよびpetunidin 3RG5Gはともに水溶液のpHに関係なく、isovitexinとのコピグメンテーションにより安定化が促進された。\n3.シアニック系アシル型品種「花籬」の花蕾からの酵素抽出物はパラ・クマロイルCoAからパラ・クマール酸の転移をアントシアニジン3RGおよび3RG5Gの両方へ触媒するが、しかしアントシアニジン3Gへの酵素活性は認められなかった。また、アントシアニンのアシル化は単一遺伝子の関与によるもので、しかもアシル化は非アシル化に対して優性であった。","subitem_description_language":"ja","subitem_description_type":"Abstract"},{"subitem_description":"In this study, acylated anthocyanins and anthocyanin p-coumaroyltransferase of Iris ensata Thunb. were characterized, and the following results were obtained.\n1. The anthocyanins of 130 cultivars and 13 lines and 3 wild forms were analyzed by HPLC, and these plants were classified into 16 types of major anthocyanins. Among these types, petunidin 3RG5G-malvidin 3RG5G, malvidin 3RG5G-peonidin 3RG5G, peonidin 3RG5G-cyanidin 3RG5G and peonidin 3RG-cyanidm 3RG were obtained new non-acylated types.\n2. The fading of flower color in acylated anthocyanin type cultivars and non-acylated anthocyanin ones and the in vitro stability of malvidin 3RGac5G, 3RG5G, petunidin 3RGac5G and 3RG5G due to different pH conditions and copigmentation with isovitexin were examined. The acylated anthocyanin cultivars exhibited higher flower color stability than the non-acylated anthocyanin ones. Malvidin 3RGac5G, 3RG5G, petunidin 3RGac5G and 3RG5G were not stable except at the lowest pH 3.2, although the acylated anthocyanins showed slightly more stable than the non-acylated ones.\n3. Enzyme extracts from flower buds of the acylated anthocyanin cultivar catalyzed in transfer of the p-coumaroyl moiety from p-coumaroyl-CoA to both the anthocyanidins 3RG and 3RG5G. In contrast, anthocyanidin 3G did not serve as acyl acceptors at all. Moreover, anthocyanin p-coumaroyltransferase was controlled by dominant gene, which is symbolized as Ac.","subitem_description_type":"Abstract"}]},"item_10007_description_14":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"平成11年度-平成13年度科学研究費補助金(基盤研究(C)(2))研究成果報告書","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_10007_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-06-21"}],"displaytype":"detail","filename":"yabuya.pdf","filesize":[{"value":"1.9 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"yabuya.pdf","url":"https://miyazaki-u.repo.nii.ac.jp/record/1917/files/yabuya.pdf"},"version_id":"a44865f2-0ce8-4761-b17e-4d84ab8888b2"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Iris ensata, acylated anthocyanin, acylation, anthocyanin biosynthesis, flower color breeding, genetic analysis, isovitexin, p-coumaroyltransferase, stability","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"アシル化, アシル化アントシアニン, アシル基転移酵素, アントシアニジン3RG5G, アントシアニン, コピグメンテーション, ハナショウブ, パラ・クマロイルCoA, パラ・クマール酸, 基質特異性, 安全性, 安定性, 花色育種, 遺伝分析, 酵素活性阻害, 金属イオン","subitem_subject_language":"ja","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_researcher":{"attribute_name":"研究代表者","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"藪谷, 勤","creatorNameLang":"ja"},{"creatorName":"ヤブヤ, ツトム","creatorNameLang":"ja-Kana"},{"creatorName":"Yabuya, Tsutomu","creatorNameLang":"en"}],"familyNames":[{},{},{}],"givenNames":[{},{},{}],"nameIdentifiers":[{}]}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"ハナショウブにおけるアントシアニンのアシル化機構の解明","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"ハナショウブにおけるアントシアニンのアシル化機構の解明","subitem_title_language":"ja"},{"subitem_title":"Characterization of acylated anthocyanin and anthocyanin p-coumaroyltransferase in Japanese garedn iris, Iris ensata Thunb","subitem_title_language":"en"}]},"item_type_id":"10007","owner":"5","path":["74","32"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2013-02-05"},"publish_date":"2013-02-05","publish_status":"0","recid":"1917","relation_version_is_last":true,"title":["ハナショウブにおけるアントシアニンのアシル化機構の解明"],"weko_creator_id":"5","weko_shared_id":-1},"updated":"2023-10-24T01:01:57.707947+00:00"}