{"created":"2023-05-15T09:57:47.444905+00:00","id":1634,"links":{},"metadata":{"_buckets":{"deposit":"4a21aaad-7b10-4fd4-988a-6492696ad52c"},"_deposit":{"created_by":5,"id":"1634","owners":[5],"pid":{"revision_id":0,"type":"depid","value":"1634"},"status":"published"},"_oai":{"id":"oai:miyazaki-u.repo.nii.ac.jp:00001634","sets":["72","72:30"]},"author_link":["28840","28845","9884","9886","9885","21696","9880","9881"],"control_number":"1634","item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2014-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"51","bibliographicPageStart":"44","bibliographicVolumeNumber":"105","bibliographic_titles":[{"bibliographic_title":"Cancer Science","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Hepatocyte growth factor activator inhibitor type 1 （HAI-1） is a membrane-bound serine protease inhibitor that is expressed on the surface of epithelial and carcinoma cells. On the cell surface, HAI-1 regulates membrane-anchored serine proteases, with matriptase being the most critical target. Matriptase is involved in pericellular processing of biologically active molecules, including proteaseactivated receptor-2 （PAR-2）. Previously we reported that S2-CP8 cells, a metastatic variant of the SUIT-2 human pancreatic adenocarcinoma cell line, showed markedly decreased HAI-1 expression. To assess the significance of HAI-1 loss in invasion and spontaneous metastasis of S2-CP8 cells, we established stable S2-CP8 sublines that expressed HAI-1 under the control of a tetracycline-regulated promoter. In vitro migration and invasion assays revealed inhibitory effects of HAI-1 on S2-CP8 cell migration and invasion. Matriptase activity was suppressed by the expression of HAI-1. As the enhanced invasiveness in the absence of HAI-1 was alleviated by knockdown of matriptase by 81% and of PAR-2 completely, and PAR-2 antagonist also suppressed the invasion, matriptase-mediated PAR-2 activation is involved in HAI-1 loss-induced invasion of S2-CP8 cells. We then analyzed the effect of HAI-1 expression on metastasis of S2-CP8 cells in vivo using a nude mouse orthotopic xenograft model. Although approximately 50% of the control mice developed distant metastasis, mice treated with doxycycline to induce HAI-1 expression did not develop metastasis. These data indicate that HAI-1 loss contributes to invasion and dissemination of a highly metastatic subline of SUIT-2, suggesting crucial roles for the balance of pericellular serine proteases/inhibitors in pancreatic cancer progression.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Japanese Cancer Association","subitem_publisher_language":"en"},{"subitem_publisher":"日本癌学会","subitem_publisher_language":"ja"}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1111/cas.12306","subitem_relation_type_select":"DOI"}}]},"item_10001_relation_18":{"attribute_name":"他の資源との関係","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"http://hdl.handle.net/10458/4878"}]}]},"item_10001_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"(C) 2013 The Authors. Cancer Science published by Wiley Publishing Asia Pty Ltd on behalf of Japanese Cancer Association.","subitem_rights_language":"en"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"叶, 景佳","creatorNameLang":"ja"},{"creatorName":"Ye, Jingjia","creatorNameLang":"en"}],"familyNames":[{"familyName":"叶","familyNameLang":"ja"},{"familyName":"Ye","familyNameLang":"en"}],"givenNames":[{"givenName":"景佳","givenNameLang":"ja"},{"givenName":"Jingjia","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"9880","nameIdentifierScheme":"WEKO"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"0000000106573887","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"https://isni.org/isni/0000000106573887"}],"affiliationNames":[{"affiliationName":"宮崎大学","affiliationNameLang":"ja"},{"affiliationName":"University of Miyazaki","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"川口, 真紀子","creatorNameLang":"ja"},{"creatorName":"カワグチ, マキコ","creatorNameLang":"ja-Kana"},{"creatorName":"Kawaguchi, 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Yukihiro","creatorNameLang":"en"}],"familyNames":[{"familyName":"春山","familyNameLang":"ja"},{"familyName":"ハルヤマ","familyNameLang":"ja-Kana"},{"familyName":"Haruyama","familyNameLang":"en"}],"givenNames":[{"givenName":"幸洋","givenNameLang":"ja"},{"givenName":"ユキヒロ","givenNameLang":"ja-Kana"},{"givenName":"Yukihiro","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"21696","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"金丸, 愛","creatorNameLang":"ja"},{"creatorName":"カネマル, アイ","creatorNameLang":"ja-Kana"},{"creatorName":"Kanemaru, 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コウジ","creatorNameLang":"ja-Kana"}],"familyNames":[{"familyName":"Yamamoto","familyNameLang":"en"},{"familyName":"山本","familyNameLang":"ja"},{"familyName":"ヤマモト","familyNameLang":"ja-Kana"}],"givenNames":[{"givenName":"Koji","givenNameLang":"en"},{"givenName":"晃士","givenNameLang":"ja"},{"givenName":"コウジ","givenNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"9885","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Lin, 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model"],"weko_creator_id":"5","weko_shared_id":-1},"updated":"2025-01-09T00:50:44.639024+00:00"}