{"created":"2023-05-15T09:57:47.444905+00:00","id":1634,"links":{},"metadata":{"_buckets":{"deposit":"4a21aaad-7b10-4fd4-988a-6492696ad52c"},"_deposit":{"created_by":5,"id":"1634","owners":[5],"pid":{"revision_id":0,"type":"depid","value":"1634"},"status":"published"},"_oai":{"id":"oai:miyazaki-u.repo.nii.ac.jp:00001634","sets":["72","72:30"]},"author_link":["9880","9881","21696","28840","9884","9885","28845","9886"],"control_number":"1634","item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2014-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"51","bibliographicPageStart":"44","bibliographicVolumeNumber":"105","bibliographic_titles":[{"bibliographic_title":"Cancer Science","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Hepatocyte growth factor activator inhibitor type 1 （HAI-1） is a membrane-bound serine protease inhibitor that is expressed on the surface of epithelial and carcinoma cells. On the cell surface, HAI-1 regulates membrane-anchored serine proteases, with matriptase being the most critical target. Matriptase is involved in pericellular processing of biologically active molecules, including proteaseactivated receptor-2 （PAR-2）. Previously we reported that S2-CP8 cells, a metastatic variant of the SUIT-2 human pancreatic adenocarcinoma cell line, showed markedly decreased HAI-1 expression. To assess the significance of HAI-1 loss in invasion and spontaneous metastasis of S2-CP8 cells, we established stable S2-CP8 sublines that expressed HAI-1 under the control of a tetracycline-regulated promoter. In vitro migration and invasion assays revealed inhibitory effects of HAI-1 on S2-CP8 cell migration and invasion. Matriptase activity was suppressed by the expression of HAI-1. As the enhanced invasiveness in the absence of HAI-1 was alleviated by knockdown of matriptase by 81% and of PAR-2 completely, and PAR-2 antagonist also suppressed the invasion, matriptase-mediated PAR-2 activation is involved in HAI-1 loss-induced invasion of S2-CP8 cells. We then analyzed the effect of HAI-1 expression on metastasis of S2-CP8 cells in vivo using a nude mouse orthotopic xenograft model. Although approximately 50% of the control mice developed distant metastasis, mice treated with doxycycline to induce HAI-1 expression did not develop metastasis. These data indicate that HAI-1 loss contributes to invasion and dissemination of a highly metastatic subline of SUIT-2, suggesting crucial roles for the balance of pericellular serine proteases/inhibitors in pancreatic cancer progression.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Japanese Cancer Association","subitem_publisher_language":"en"},{"subitem_publisher":"日本癌学会","subitem_publisher_language":"ja"}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1111/cas.12306","subitem_relation_type_select":"DOI"}}]},"item_10001_relation_18":{"attribute_name":"他の資源との関係","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"http://hdl.handle.net/10458/4878"}]}]},"item_10001_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"(C) 2013 The Authors. Cancer Science published by Wiley Publishing Asia Pty Ltd on behalf of Japanese Cancer Association.","subitem_rights_language":"en"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"叶, 景佳","creatorNameLang":"ja"},{"creatorName":"Ye, Jingjia","creatorNameLang":"en"}],"familyNames":[{},{}],"givenNames":[{},{}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kawaguchi, Makiko","creatorNameLang":"en"},{"creatorName":"川口, 真紀子","creatorNameLang":"ja"},{"creatorName":"カワグチ, マキコ","creatorNameLang":"ja-Kana"}],"familyNames":[{},{},{}],"givenNames":[{},{},{}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"春山, 幸洋","creatorNameLang":"ja"},{"creatorName":"ハルヤマ, ユキヒロ","creatorNameLang":"ja-Kana"},{"creatorName":"Haruyama, Yukihiro","creatorNameLang":"en"}],"familyNames":[{},{},{}],"givenNames":[{},{},{}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"金丸, 愛","creatorNameLang":"ja"},{"creatorName":"カネマル, アイ","creatorNameLang":"ja-Kana"},{"creatorName":"Kanemaru, Ai","creatorNameLang":"en"}],"familyNames":[{},{},{}],"givenNames":[{},{},{}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Fukushima, Tsuyoshi","creatorNameLang":"en"},{"creatorName":"福島, 剛","creatorNameLang":"ja"},{"creatorName":"フクシマ, ツヨシ","creatorNameLang":"ja-Kana"}],"familyNames":[{},{},{}],"givenNames":[{},{},{}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Yamamoto, Koji","creatorNameLang":"en"},{"creatorName":"山本, 晃士","creatorNameLang":"ja"},{"creatorName":"ヤマモト, コウジ","creatorNameLang":"ja-Kana"}],"familyNames":[{},{},{}],"givenNames":[{},{},{}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Lin, Chen-Yong","creatorNameLang":"en"}],"familyNames":[{}],"givenNames":[{}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kataoka, Hiroaki","creatorNameLang":"en"},{"creatorName":"片岡, 寛章","creatorNameLang":"ja"},{"creatorName":"カタオカ, ヒロアキ","creatorNameLang":"ja-Kana"}],"familyNames":[{},{},{}],"givenNames":[{},{},{}],"nameIdentifiers":[{},{}]}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"HAI-1, invasion, metastasis, pancreatic cancer, PAR-2","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Loss of hepatocyte growth factor activator inhibitor type 1 participates in metastatic spreading of human pancreatic cancer cells in a mouse orthotopic transplantation model","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Loss of hepatocyte growth factor activator inhibitor type 1 participates in metastatic spreading of human pancreatic cancer cells in a mouse orthotopic transplantation model","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"5","path":["72","30"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2014-06-10"},"publish_date":"2014-06-10","publish_status":"0","recid":"1634","relation_version_is_last":true,"title":["Loss of hepatocyte growth factor activator inhibitor type 1 participates in metastatic spreading of human pancreatic cancer cells in a mouse orthotopic transplantation model"],"weko_creator_id":"5","weko_shared_id":-1},"updated":"2024-02-08T06:14:46.739410+00:00"}