{"created":"2023-07-29T23:08:38.139912+00:00","links":{},"metadata":{"_buckets":{"deposit":"73adca1a-10a0-4ad0-9392-83e8eba90396"},"_deposit":{"created_by":9,"id":"1415.3","owner":"5","owners":[5],"pid":{"revision_id":0,"type":"depid","value":"1415.3"},"status":"published"},"_oai":{"id":"oai:miyazaki-u.repo.nii.ac.jp:00001415.3","sets":["72","72:30"]},"author_link":["8016","8228","7727","8229","7730","8383"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2006-05","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"40","bibliographicPageStart":"33","bibliographicVolumeNumber":"17","bibliographic_titles":[{"bibliographic_title":"てんかん治療研究振興財団研究年報","bibliographic_titleLang":"ja"},{"bibliographic_title":"Annual report of the Japan Epilepsy Research Foundation","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"グルタミン酸-GABAシナプス伝達は再取り込みによって調整されるため, 各トランスポーター機能はてんかん性病態における興奮 - 抑制系間の均衡に影響すると考えられる｡ グルタミン酸トランスポーター機能調整因子（GTRAP3-18）は, グルタミン酸とEAAC-1の結合性を制御する蛋白であり, さらにEAAC-1経由でGABAニューロン内に取り込まれるグルタミン酸からのGABA再合成を修飾していることから, GTRAP3-18の役割解明はてんかん研究において支持されてきた興奮系 - 抑制系間不均衡仮説の解釈を深めていくうえで,  重要なテーマである。PTZキンドリング完成後には, GTRAP3-18発現低下の長期持続が特徴的であったため, アンチセンス法を用いてGTRAP3-18ノックダウン操作を行い, てんかん原性獲得･けいれん変化およびグルタミン酸･GABA動態を観察した｡ その結果GTRAP3-18ノックダウン群ではけいれん閾値の低下とキンドリングの早期完成とともに, 海馬グルタミン酸･GABA濃度の基礎値上昇が観察された｡GABA基礎値上昇は, GTRAP3-18発現低下によってEAAC-1経由のグルタミン酸再取り込みが昂進し, GABA合成能が促進された帰結であると考察した。\n\\nAs well known the knockdown EAAC-1 by anti-sense induces epileptic convulsion in rats, EAAC-1 is important protein to connect glutamate re-uptake with GABA synthesis. It is important study to elucidate the role of glutamate transporter associated protein 3-18 （GTRAP3-18）, because GTRAP3-18 inhibitory regulates the glutamate re-uptake through EAAC-1 into GABAergic neurons. In this study, suppression of GTRAP3-18 protein expression was long-lasted after PTZ kindling, and GTRAP3-18 knockdown by anti-sense method decreases seizure threshold and accelerates the kindling phenomena. Hippocampal glutamate and GABA basal release in GTRAP3-18 knockdown group higher rather than those of senseinjected group suggested knockdown of GTRAP3-18 promotes GABA synthesis. Sustenance of high seizure susceptibility in the PTZ kindled state and the existence of inter-paroxysmal period could be explained by synchronization of glutamatergic neuron caused by the enhanced GABAergic system.","subitem_description_language":"ja","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"てんかん治療研究振興財団","subitem_publisher_language":"ja"}]},"item_10001_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN10152636","subitem_source_identifier_type":"NCID"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"09155902","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"植田, 勇人","creatorNameLang":"ja"},{"creatorName":"ウエダ, ユウト","creatorNameLang":"ja-Kana"},{"creatorName":"Ueda, Yuto","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"土井, 拓","creatorNameLang":"ja"},{"creatorName":"ドイ, タク","creatorNameLang":"ja-Kana"},{"creatorName":"Doi, Taku","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"中島, 暉","creatorNameLang":"ja"},{"creatorName":"ナカジマ, アキラ","creatorNameLang":"ja-Kana"},{"creatorName":"Nakajima, Akira","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"徳丸, 潤","creatorNameLang":"ja"},{"creatorName":"トクマル, ジュン","creatorNameLang":"ja-Kana"},{"creatorName":"Tokumaru, Jun","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"鶴, 紀子","creatorNameLang":"ja"},{"creatorName":"ツル, ノリコ","creatorNameLang":"ja-Kana"},{"creatorName":"Tsuru, Noriko","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"石田, 康","creatorNameLang":"ja"},{"creatorName":"イシダ, ヤスシ","creatorNameLang":"ja-Kana"},{"creatorName":"Ishida, Yasushi","creatorNameLang":"en"}],"nameIdentifiers":[{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-06-21"}],"displaytype":"detail","filename":"5-6a_ueda-tenkannenpo-17-2006.pdf","filesize":[{"value":"993.8 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"5-6a_ueda-tenkannenpo-17-2006.pdf","url":"https://miyazaki-u.repo.nii.ac.jp/record/1415/files/5-6a_ueda-tenkannenpo-17-2006.pdf"},"version_id":"7c980510-278a-4528-b0cb-44cdc273a78a"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"epilepsy, glutamate, GABA, glutamate transporter associated protein 3-18, western blot","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"キンドリング形成過程におけるグルタミン酸トランスポーター機能調節因子(GTRAP3-18)の機能解明とGABA合成能に関する研究","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"キンドリング形成過程におけるグルタミン酸トランスポーター機能調節因子(GTRAP3-18)の機能解明とGABA合成能に関する研究","subitem_title_language":"ja"},{"subitem_title":"The functional role of glutamate transporter associated protein (GTRAP3-18) in the epileptogenesis induced by PTZ-kindling","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"5","path":["72","30"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2009-07-29"},"publish_date":"2009-07-29","publish_status":"0","recid":"1415.3","relation_version_is_last":true,"title":["キンドリング形成過程におけるグルタミン酸トランスポーター機能調節因子(GTRAP3-18)の機能解明とGABA合成能に関する研究"],"weko_creator_id":"5","weko_shared_id":2},"updated":"2024-01-30T04:23:09.464929+00:00"}