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Antiparallel RNA G-quadruplex Formed by Human Telomere RNA Containing 8-Bromoguanosine 肖, 潮達 ショウ, チョウタツ Xiao, Chao-Da 肖 ショウ Xiao 潮達 チョウタツ Chao-Da 50700085 石塚, 匠 イシヅカ, タクミ Ishizuka, Takumi 石塚 イシヅカ Ishizuka 匠 タクミ Takumi 宮崎大学 University of Miyazaki 40506763 徐, 岩 ジョ, ガン Xu, Yan 徐 ジョ Xu 岩 ガン Yan 宮崎大学 University of Miyazaki open access © The Author(s) 2017 In this study, by combining nuclear magnetic resonance (NMR), circular dichroism (CD), liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS), and gel electrophoresis, we report an unusual topological structure of the RNA G-quadruplex motif formed by human telomere RNA r(UAGGGU) containing 8-bromoguanosine. Results showed that the RNA sequence formed an antiparallel tetramolecular G-quadruplex, in which each pair of diagonal strands run in opposite directions. Furthermore, guanosines were observed both in syn- and anti-conformations. In addition, two of these G-quadruplex subunits were found to be stacking on top of each other, forming a dimeric RNA G-quadruplex. Our findings provide a new insight into the behavior of RNA G-quadruplex structures. Citation: Xiao CD, Ishizuka T, Xu Y. Antiparallel RNA G-quadruplex Formed by Human Telomere RNA Containing 8-Bromoguanosine. Sci Rep. 2017 Jul 27;7(1):6695. doi: 10.1038/s41598-017-07050-w. PMID: 28751647; PMCID: PMC5532209. Springer Nature 2017-07-27 eng journal article VoR http://hdl.handle.net/10458/0002000447 https://miyazaki-u.repo.nii.ac.jp/records/2000447 https://doi.org/10.1038/s41598-017-07050-w Scientific Reports 7 1 6695 https://miyazaki-u.repo.nii.ac.jp/record/2000447/files/Xiao2_2018.pdf application/pdf 2.7 MB 2024-01-25