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Factors from fluid of the ovarian pocket that stimulate sperm motility in domestic hens fowl sperm motility regulation Ashizawa, Koji 芦沢, 幸二 アシザワ, コウジ Ashizawa 芦沢 アシザワ Koji 幸二 コウジ Wishart, G.J. 精子 sperm, spermatozoa, motility, domestic fowl, fertilization About 5ml of fluid could be collected from the region of the peritoneum surrounding the ovarian pocket of domestic fowl hens only around the time of ovulation. This fluid stimulated the motility of fowl spermatozoa 6-fold at 40℃ in vitro. Gel filtration of the revealed 2 peaks of motility-stimulating activity: one of these was identified as calcium; the other as a low molecular weight (Mr 200) heat-labile substance. From evidence, which it discussed, it is suggested that this fluid is representative of the milieu which surrounds the ovum at the time of fertilization ; and thus that the motility-stimulating factors may be implicated in promoting fertilization by activating spermatozoa, which are normally stored in a quiescent state within the oviduct. Disclaimer. This is not the definitive version of record of this article This manuscript has been accepted for publication in Reproduction, but the version presented here has not yet been copy edited, formatted or proofed. Consequently, neither Reproduction, the Society for Reproduction and Fertility nor its publisher, BioScientifica Ltd, accepts responsibility for any errors or omissions it may contain. The definitive version is now freely available at [insert DOI link]. 1992 Society for Reproduction and Fertility. 1992 eng journal article AM http://hdl.handle.net/10458/705 https://miyazaki-u.repo.nii.ac.jp/records/461 AA00705952 00224251 Journal of reproduction and fertility 95 3 855 860 https://miyazaki-u.repo.nii.ac.jp/record/461/files/ashizawa40.pdf application/pdf 775.0 kB 2020-06-21