2026-03-13T20:21:32Z https://miyazaki-u.repo.nii.ac.jp/oai

oai:miyazaki-u.repo.nii.ac.jp:00000845 2025-07-23T10:14:46Z 74 74:26

Inhibition of Flagellar Motility of Fowl Spermatozoa by L-Carnitine: Its Relationship with Respiration and Phosphorylation of Axonemal Proteins Carnitine and sperm motility Ashizawa, Koji 芦沢, 幸二 アシザワ, コウジ Ashizawa 芦沢 アシザワ Koji 幸二 コウジ Kamiya, Tetsushi Tamura, Ikue 00236928 續木, 靖浩 ツヅキ, ヤスヒロ Tsuzuki, Yasuhiro 續木 ツヅキ Tsuzuki 靖浩 ヤスヒロ Yasuhiro 宮崎大学 University of Miyazaki 精子 sperm, Demembranated sperm, Dynein, Calcium, Oxygen consumption The action of carnitine in regulating fowl sperm motility was investigated. As the concentration of L-carnitine was increased (0-20 mM), the motility of intact and demembranated fowl spermatozoa was reduced at 30°C. Even the presence of 1 mM CaCl2 before the addition of 10 mM carnitine could not prevent the inhibition of motility at 30°C and 40°C. However, motility was restored by reducing the concentrations of carnitine. Carnitine also inhibited the oxygen consumption and ATP concentrations of intact spermatozoa, and caused a reduction in intracellular free Ca2+ concentrations. Phosphorylation of a 50 kDa protein and dephosphorylation of 24 kDa and 30 kDa proteins of demembranated spermatozoa were observed after the addition of carnitine. In contrast, the flagellar ATPase activity of crude dynein extract was not affected by the addition of carnitine. These results suggest that inhibitory effect of carnitine for motility may be directly on the axonemal phosphoproteins, but not directly on the dynein ATPase activity. The physiological role of carnitine for fowl spermatozoa in the ductus deferens is discussed. 1994 eng journal article AM http://hdl.handle.net/10458/733 https://miyazaki-u.repo.nii.ac.jp/records/845 AA10735246 1040452X Molecular reproduction and development 38 3 318 325 https://miyazaki-u.repo.nii.ac.jp/record/845/files/ashizawa50.pdf application/pdf 1.6 MB 2020-06-21