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REGULATORY SYSTEM OF TEMPERATURE-DEPENDENT FLAGELLAR MOTILITY IS RETAINED AFTER FREEZING AND THAWING OF DEMEMBRANATED FOWL SPERMATOZOA 芦沢, 幸二 Ashizawa, Koji Okabe, H. Tsuzuki, Y. 精子 sperm, spermatozoa, motility, freezing, temperature, fowl In the presence of adenosine 5'-triphosphate （ATP）, the motility of demembranated fowl spermatozoa was vigorous at 30°C, both before and after rapid freezing at -70°C or -196°C without cryoprotectants. The rate of ATP consumption of the demembranated spermatozoa treated by freezing and thawing was, at approximately 200 nmol ATP hydrolysis/109 spermatozoa/min, also similar to that of the untreated spermatozoa. At 40°C, neither frozen- thawed spermatozoa nor control spermatozoa were motile, but motility could be restored by decreasing the temperature to 30°C or by the addition of inhibitors of protein phosphatases. These observations suggest that the substance（s） involved in the temperature-dependent immobilization of fowl spermatozoa are closely associated with the axoneme, and that physical damage to the demembranated spermatozoa, such as freezing and thawing, is not enough to remove the substance（s）. 1996 eng journal article AM http://hdl.handle.net/10458/720 https://miyazaki-u.repo.nii.ac.jp/records/307 AA00862972 0093691X Theriogenology : an international journal of animal reproduction 46 8 1413 1424 https://miyazaki-u.repo.nii.ac.jp/record/307/files/ashizawa57.pdf application/pdf 1.1 MB 2020-06-21