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Aspergillus niger No.12株の細胞内エキソおよびエンド型イヌリナーゼの精製と性質
http://hdl.handle.net/10458/1788
http://hdl.handle.net/10458/1788c2be206e-8c29-449e-a4ea-bc4095ff670e
名前 / ファイル | ライセンス | アクション |
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KJ00000503394.pdf (461.3 kB)
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2009-02-03 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Purification and Properties of Intracellular Exo- and Endoinlulinases from Aspergillus niger Strain 12 | |||||
タイトル | ||||||
言語 | ja | |||||
タイトル | Aspergillus niger No.12株の細胞内エキソおよびエンド型イヌリナーゼの精製と性質 | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Aspergillus niger, Intracellular enzyme, Inulin, Inulinase | |||||
キーワード | ||||||
言語 | ja | |||||
主題 | 細胞内酵素, イヌリン, イヌリナーゼ | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
著者 |
中村, 豊彦
× 中村, 豊彦× 倉盛, 宏一× 在田, 典弘× 秋元, 秀俊× 太田, 一良× Kuramori, Koichi× 在田, 典弘× 秋元, 秀俊 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Exo- and endoinulinases were extracted from mycelia of Aspergillus niger strain 12 using quartz sand and purified by DEAE-Cellulofine A-500, Sephadex G-1OO, and Sephadex G-200 chromatographies. The intracellular enzymes were homogeneous as judged by SDS-polyacrylamide gel electrophoresis. The purified exoinulinase P-II had specific activities of 6.6 U/mg toward inulin and 22 U/mg toward sucrose. The endoinulinase P-III showed 108 U/mg toward inulin, but no activity toward sucrose. Mrs of exoinulinase P-II and endoinulinase P-III were determined by gel filtration using Sephadex G-200 as 47 and 56 kDa, respectively. Optimal pH and temperature for enzyme activity were pH-5.0 and 55 ℃ for P-II, and pH 5.3 and 45℃ for P-III. Both the enzymes were activated by Mn2+ , and inactivated by Ag+, Hg2+, or p-chloromercuribenzoate. Inulinases P-II and P-III exhibited apparent Km values of 5.8 and 0.80 mM, respectively. |
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言語 | en | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | 黒麹菌Aspergillus nigerNo.12株の培養菌体を石英砂で破砕し,その抽出液から細胞内エキソおよびエンド型イヌリナーゼをDEAE-セルロファインA-500,続いてセファデックスG-100およびG-200クロマトグラフィーによりそれぞれ電気泳動的に単一に精製した。エキソ型イヌリナーゼP-IIの比活性は,イヌリンに対して6.6U/mg,ショ糖に対して22U/mgであった。エンド型イヌリナーゼP-IIIはイヌリンのみに作用し,比活性は108U/mgであった。 ゲルろ過で測定した分子量は,エキゾ型イヌリナーゼP-IIが47kDa,エンド型イヌリナーゼP-IIIが56kDaであった。エキソ型イヌリナーゼP-IIとエンド型イヌリナーゼP-IIIの最適pHはそれぞれpH5.0とpH5.3,最適温度はそれぞれ55℃と45℃であった。両酵素ともMn2+で賦活化され,Ag+,Hg2+,p-クロロメルクリ安息香酸で失活した。エキソ型イヌリナーゼP-IIとエンド型イヌリナーゼP-IIIのイヌリンに対するMichaelis定数(Km)は,それぞれ5.8mMと0.80mMであった。 |
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言語 | ja | |||||
書誌情報 |
ja : 宮崎大学農学部研究報告 en : Bulletin of the Faculty of Agriculture, Miyazaki University 巻 48, 号 1-2, p. 49-58, 発行日 2001-12 |
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出版者 | ||||||
言語 | ja | |||||
出版者 | 宮崎大学農学部 | |||||
出版者 | ||||||
言語 | en | |||||
出版者 | Miyazaki University | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 05446066 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AN00236503 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |