Chimeric simian and human immunodeficiency viruses (SHIVs) are useful tool for investigating AIDS pathogenesis and for development of vaccine. We constructed a SHIV-vpr vector (designated as SHIV-3sj) by replacing vpr region with restriction enzyme sites. SHIV-3sj was designed to express inserted gene along with its viral replication. Five cytokine genes were inserted into SHIV-3sj, and ability of viral replication and expression of the inserted genes were examined. The short insert including RANTES and IL-5 resulted in the successful expression from SHIV-3sj, while the construct having longer genes including IL-2, IL-6 and IL-12p35 failed to become replication competent. These results suggest that the length of the insert is an important factor for the replication ability of SHIV-3sj vector.
SIV/HIVキメラウイルス(SHIV)のvpr領域を制限酵素サイトに置換し,SHIV-3sjベクターを作成した.5種のサイトカイン遺伝子をSHIV3sjに挿入した結果,RANTESやIL-5といった短い遺伝子は発現が確認されたが,IL-2,IL-6,IL-12p35といった長い遺伝子を挿入したものは複製能を欠いた.挿入遺伝子の長さがSHIV-3sjベクターの複製を規定している可能性が示唆された.
リポジトリのご案内
Characterization of vpr Vector Constructed from Chimeric Simian and Human Immunodeficiency Virus
2881haga.pdf
(62.02KB)
